BRAT1抗体;BRAT1 Antibody—艾普蒂

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     Western blot analysis of BRAT1 expression in HeLa cell lysate.    

  
  

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     Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)    

  
  

The BRAT1 (BRCA1-associated ATM activator 1) gene encodes a protein involved in DNA damage response, cell cycle regulation, and maintenance of genomic stability. It interacts with key DNA repair proteins, including BRCA1 and ATM kinase, playing a critical role in mediating double-strand break repair and cellular survival. BRAT1 mutations are linked to severe neurodevelopmental disorders, such as rigidity and multifocal seizure syndrome (RMFS1) and early-onset epileptic encephalopathy, highlighting its importance in neuronal development and function.

BRAT1 antibodies are essential tools for studying these biological processes. They enable the detection and quantification of BRAT1 protein expression in tissues or cultured cells through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers use these antibodies to explore BRAT1's role in cancer biology, particularly its interaction with BRCA1 in DNA repair mechanisms, as well as its implications in neurodevelopmental diseases. Commercially available BRAT1 antibodies vary in clonality (monoclonal or polyclonal) and epitope specificity, requiring validation for experimental reproducibility. Recent studies also investigate BRAT1's potential as a biomarker or therapeutic target, emphasizing the antibody's utility in both basic research and translational applications. Proper controls, such as knockout cell lines, are recommended to confirm antibody specificity due to overlapping functions with related DNA repair proteins.