ALIX抗体;ALIX Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

ALIX (ALG-2-interacting protein X), also known as PDCD6IP, is a multifunctional adaptor protein involved in membrane remodeling and intracellular trafficking processes. It was initially identified through its interaction with ALG-2. a calcium-binding protein linked to apoptosis. ALIX plays a critical role in the endosomal sorting complex required for transport (ESCRT) pathway, facilitating membrane scission events during cytokinesis, multivesicular body (MVB) formation, viral budding, and plasma membrane repair. Its modular structure, featuring Bro1. V, and PRR domains, enables interactions with ESCRT-III components, ubiquitinated cargoes, and viral proteins like HIV-1 Gag.

Antibodies targeting ALIX are essential tools for studying these processes. They are widely used in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to investigate ALIX’s localization, protein interactions, and regulatory mechanisms. Commercial ALIX antibodies are often raised against specific epitopes (e.g., human ALIX residues 1-150 or 750-868) and validated for species cross-reactivity (human, mouse, rat). Researchers employ these antibodies to explore ALIX’s roles in diseases, including cancer (metastasis suppression), neurodegenerative disorders (exosome-mediated protein aggregation), and viral pathogenesis (e.g., HIV-1 release). Variability in antibody performance across applications underscores the importance of rigorous validation for experimental reproducibility.