GCLM抗体;GCLM Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of GCLM expression in K562 cell lysate.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:25 dilution.    

  
  

The glutamate-cysteine ligase modifier subunit (GCLM) is a critical regulatory component of the glutamate-cysteine ligase (GCL) enzyme, which catalyzes the rate-limiting step in glutathione (GSH) biosynthesis. GCL functions as a heterodimer, comprising a catalytic subunit (GCLC) and a modifier subunit (GCLM). GCLM enhances the catalytic efficiency of GCLC by lowering its Km for ATP and increasing its Ki for GSH feedback inhibition, thereby fine-tuning cellular antioxidant capacity. As glutathione is the primary intracellular antioxidant, GCLM plays a pivotal role in maintaining redox homeostasis and defending against oxidative stress.

GCLM antibodies are immunological tools developed to detect, quantify, and study the expression, localization, and function of the GCLM protein. These antibodies are widely utilized in research exploring oxidative stress-related pathologies, including neurodegenerative diseases (e.g., Parkinson’s, Alzheimer’s), cardiovascular disorders, cancer, and metabolic syndromes. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess GCLM levels in tissues or cultured cells under varying oxidative conditions.

Studies using GCLM antibodies have revealed its involvement in adaptive responses to environmental toxins, inflammation, and chemotherapeutic agents. Polymorphisms in the GCLM gene, linked to reduced GSH synthesis, are associated with increased disease susceptibility, making GCLM a potential biomarker for oxidative stress-related conditions. Research continues to explore therapeutic strategies targeting GCLM to modulate cellular antioxidant defenses, highlighting the antibody’s importance in both mechanistic and translational investigations.