ANP抗体;ANP Antibody—艾普蒂

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     Western blot analysis of ANP expression in PC3 cell lysate.    

  
  

Atrial natriuretic peptide (ANP) antibodies are immunological tools used to detect and quantify ANP, a cardiac hormone primarily secreted by atrial cardiomyocytes in response to stretching of the atrial walls. ANP plays a critical role in regulating blood pressure, fluid balance, and sodium excretion by promoting vasodilation, diuresis, and inhibition of the renin-angiotensin-aldosterone system. Research on ANP antibodies emerged alongside discoveries of ANP's physiological roles in the 1980s, driven by the need to understand its involvement in cardiovascular and renal diseases.

These antibodies are typically developed in laboratory animals (e.g., rabbits, mice) using synthetic ANP fragments as antigens. They enable precise detection methods like ELISA, radioimmunoassay, and immunohistochemistry, facilitating studies on ANP expression, secretion patterns, and receptor interactions. Clinically, ANP antibodies aid in diagnosing heart failure and hypertension by measuring circulating ANP levels, which often correlate with disease severity.

Recent applications extend to exploring ANP's anti-inflammatory and metabolic effects, including its role in obesity and diabetes. Challenges remain in ensuring antibody specificity due to structural similarities between ANP and related natriuretic peptides (BNP, CNP). Ongoing research focuses on refining antibody-based assays and therapeutic strategies targeting ANP pathways.