Phospho-PAK4+PAK5+PAK6(S474+S560+S602)抗体;Phospho-PAK4+PAK5+PAK6(S474+S560+S602) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) expression in U87-MG cell lysate.    

  
  

The Phospho-PAK4+PAK5+PAK6 (S474+S560+S602) antibody is designed to detect the activated (phosphorylated) forms of p21-activated kinases 4. 5. and 6. which belong to the Group II PAK family. These serine/threonine kinases act as downstream effectors of Rho GTPases (e.g., Cdc42. Rac1) and regulate diverse cellular processes, including cytoskeletal reorganization, cell motility, survival, and transcriptional activation. The antibody specifically recognizes phosphorylation at Ser474 in PAK4. Ser560 in PAK5. and Ser602 in PAK6 – sites critical for their kinase activation and downstream signaling.

Phosphorylation at these residues is associated with conformational changes that relieve autoinhibition, enabling PAK interaction with substrates or binding partners. Aberrant PAK4/5/6 activation has been implicated in cancer progression, metastasis, and resistance to therapy, making their phosphorylated forms valuable biomarkers for studying oncogenic pathways. In neurological contexts, these kinases influence synaptic plasticity and neuronal development, with dysregulation linked to neurodegenerative disorders.

This antibody is widely used in Western blotting, immunofluorescence, or immunoprecipitation to investigate PAK activation dynamics in response to extracellular stimuli (e.g., growth factors, stress) or pathological conditions. Researchers often pair it with pan-PAK antibodies to assess activation ratios. However, cross-reactivity between PAK isoforms requires validation via knockout controls or isoform-specific assays. Its multiplex detection capability makes it a practical tool for comparative studies of Group II PAKs in overlapping or distinct signaling contexts.