PAK1抗体;PAK1 Antibody—艾普蒂

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     Western blot analysis of PAK1 expression in A431 cell lysate.    

  
  

The PAK1 (p21-activated kinase 1) antibody is a key tool for studying the role of PAK1. a serine/threonine kinase belonging to the PAK family. PAK1 is activated by binding to Rho GTPases like Cdc42 and Rac, which triggers conformational changes and autophosphorylation, enabling its kinase activity. It regulates diverse cellular processes, including cytoskeletal reorganization, cell motility, proliferation, survival, and gene expression. PAK1 dysregulation is implicated in cancer progression, neurological disorders, and infectious diseases, making it a significant therapeutic target.

PAK1 antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to detect PAK1 expression, localization, and activation states in cells or tissues. Specific antibodies may target distinct regions of PAK1. such as its kinase domain or regulatory motifs, to study functional interactions or phosphorylation events (e.g., at Thr423). Researchers also utilize PAK1 antibodies to explore its signaling crosstalk with pathways like MAPK/ERK and PI3K/AKT, as well as its role in tumor invasiveness, synaptic plasticity, and pathogen-host interactions. Commercial PAK1 antibodies vary in host species, clonality, and validation data, necessitating careful selection based on experimental requirements.