PKCepsilon抗体;PKCepsilon Antibody—艾普蒂

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     Western blot analysis of PKC epsilon expression in Jurkat cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

The protein kinase C epsilon (PKCε) antibody is a crucial tool in biomedical research, targeting the PKCε isoform, a member of the serine/threonine kinase family. PKCε belongs to the novel PKC subfamily, characterized by its calcium-independent activation and dependence on diacylglycerol (DAG) or phorbol esters. It plays diverse roles in cellular signaling, regulating processes like proliferation, differentiation, apoptosis, and stress responses. Dysregulation of PKCε has been implicated in cancer, neurodegenerative diseases, cardiovascular disorders, and metabolic syndromes, making it a focal point for therapeutic and mechanistic studies.

PKCε antibodies are widely used to detect endogenous PKCε expression and localization in tissues or cultured cells via techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies are typically raised in hosts like rabbits or mice, with specificity validated through knockout controls or siRNA-mediated silencing. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple epitopes, enhancing sensitivity for low-abundance targets.

Researchers rely on PKCε antibodies to explore its structural domains, including the N-terminal regulatory region (C1. C2-like domains) and the C-terminal catalytic kinase domain. Commercial antibodies often cite applications in cancer research (e.g., tumor invasion), neuroscience (e.g., synaptic plasticity), and cardiology (e.g., ischemic preconditioning). Variability in antibody performance across vendors underscores the need for rigorous validation in specific experimental contexts. Overall, PKCε antibodies remain indispensable for unraveling the isoform-specific functions of this multifaceted kinase.