Apg12(Atg12)抗体;Apg12(Atg12) Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Apg12(Atg12) expression in 293 cell lysate.    

  
  

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     Pyrroloquinoline quinine ameliorates doxorubicin-induced autophagy-dependent apoptosis via lysosomal-mitochondrial axis in vascular endothelial cells. -Toxicology    

  
  

The Atg12 (Autophagy-related protein 12) antibody is a crucial tool for studying the molecular mechanisms of autophagy, a conserved cellular process responsible for degrading damaged organelles, misfolded proteins, and pathogens. Atg12 is a key component of the ubiquitin-like conjugation system essential for autophagosome formation. During autophagy, Atg12 covalently binds to Atg5 via an E1/E2-like enzymatic cascade involving Atg7 and Atg10. forming the Atg12-Atg5 conjugate. This complex further interacts with Atg16L1 to promote autophagosome membrane elongation and LC3 lipidation, a critical step in autophagosome maturation.

The Atg12 antibody is widely used to detect endogenous Atg12 protein levels or its conjugated form (Atg12-Atg5) in various experimental models, including cell lines, tissues, and *in vivo* systems. Researchers employ this antibody in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore autophagy regulation under physiological or stress conditions (e.g., nutrient deprivation, oxidative stress, or infection). It also aids in studying autophagy-related diseases, such as cancer, neurodegenerative disorders (e.g., Alzheimer’s), and infectious diseases, where autophagy dysregulation is implicated.

Validated for specificity and sensitivity, the Atg12 antibody helps dissect autophagy pathways and evaluate potential therapeutic interventions targeting this process. Its application has significantly advanced our understanding of autophagy’s role in cellular homeostasis, disease progression, and treatment responses.