EAAT2抗体;EAAT2 Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of EAAT2 expression in HeLa cell lysate.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

EAAT2 (Excitatory Amino Acid Transporter 2), also known as SLC1A2. is a glutamate transporter protein predominantly expressed in astrocytes, playing a critical role in regulating extracellular glutamate levels in the central nervous system. By rapidly clearing synaptic glutamate, EAAT2 prevents excessive neuronal excitation and potential excitotoxicity linked to neurodegenerative and neuropsychiatric disorders. EAAT2 dysfunction has been implicated in conditions such as amyotrophic lateral sclerosis (ALS), epilepsy, Alzheimer’s disease, and ischemic stroke.

EAAT2 antibodies are immunological tools designed to detect and study the expression, localization, and functional dynamics of this transporter. These antibodies, often monoclonal or polyclonal, are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess EAAT2 levels in tissue samples, cell cultures, or animal models. Researchers employ EAAT2 antibodies to investigate pathological mechanisms, such as downregulation or mislocalization of the transporter in disease states, or to evaluate therapeutic interventions aimed at restoring glutamate homeostasis.

Validation of EAAT2 antibodies is crucial due to potential cross-reactivity with other glutamate transporters (e.g., EAAT1 or EAAT3) or splice variants. Specificity is typically confirmed using knockout models or siRNA-mediated silencing. As glutamate dysregulation remains a therapeutic target, EAAT2 antibodies continue to serve as essential reagents in both basic neuroscience research and drug development.