PABPN1抗体;PABPN1 Antibody—艾普蒂

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     Western blot analysis of PABPN1 expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

Polyadenylate-binding protein nuclear 1 (PABPN1) is a critical protein involved in mRNA processing, primarily known for regulating poly(A) tail elongation during RNA maturation. It binds to nascent poly(A) tails synthesized by poly(A) polymerase and facilitates mRNA export from the nucleus to the cytoplasm. PABPN1 also plays roles in alternative polyadenylation, RNA stability, and transcription termination. Mutations in the PABPN1 gene, particularly expansions of a GCG trinucleotide repeat encoding an alanine tract, are linked to oculopharyngeal muscular dystrophy (OPMD), a late-onset neuromuscular disorder characterized by muscle weakness, dysphagia, and intranuclear aggregates in affected cells.

Antibodies targeting PABPN1 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect endogenous PABPN1 in tissues or cultured cells. Researchers employ PABPN1-specific antibodies to investigate its role in RNA metabolism, its aggregation behavior in OPMD models, and its interaction with other RNA-binding proteins. Commercially available antibodies are typically raised against recombinant human PABPN1 or specific epitopes, with validation including knockout cell lines or tissue controls to confirm specificity. Understanding PABPN1 dynamics through antibody-based assays contributes to insights into OPMD pathogenesis and potential therapeutic strategies.