Claudin5抗体;Claudin5 Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Claudin 5 expression in human fetal brain lysate.    

  
  

Claudin5 is a key component of tight junction complexes, primarily expressed in endothelial and epithelial cells, where it plays a critical role in regulating paracellular permeability and maintaining cellular polarity. As a member of the claudin family, it is integral to the formation of the blood-brain barrier (BBB), selectively restricting the passage of molecules between circulating blood and the central nervous system. Its structure includes four transmembrane domains, contributing to the selective "sealing" of intercellular spaces. Dysregulation of Claudin5 has been linked to various pathologies, including stroke, neurodegenerative diseases (e.g., Alzheimer's), and cancer metastasis, where BBB integrity is compromised or cellular invasion is promoted.

Antibodies targeting Claudin5 are essential tools in biomedical research, enabling the detection, localization, and functional analysis of this protein. They are widely used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to study BBB dynamics in health and disease. For instance, reduced Claudin5 expression is often observed in neuroinflammatory conditions, while its overexpression in certain tumors correlates with aggressive behavior. Researchers also utilize these antibodies to explore therapeutic strategies aimed at modulating tight junction function—for example, enhancing drug delivery across the BBB or inhibiting cancer cell dissemination. Validated Claudin5 antibodies are critical for ensuring specificity in these applications, particularly given the structural similarities among claudin family members.