CaMKIIalpha抗体;CaMKIIalpha Antibody—艾普蒂

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     Western blot analysis of CaMKII alpha expression in SH-SY5Y cell lysate.    

  
  

CaMKIIα (calcium/calmodulin-dependent protein kinase II alpha) is a key enzyme in synaptic plasticity and memory formation, predominantly expressed in the forebrain, particularly within excitatory neurons. As a major subunit of the CaMKII holoenzyme, it undergoes autophosphorylation upon calcium influx, enabling sustained kinase activity critical for long-term potentiation (LTP). Antibodies targeting CaMKIIα are widely used in neuroscience research to study its expression, localization, and activation dynamics in neural circuits. These antibodies typically recognize specific epitopes, such as phosphorylation sites (e.g., Thr286/287) or conserved regions of the kinase domain, allowing detection of active versus inactive states.

Developed in various host species (e.g., rabbit, mouse), CaMKIIα antibodies are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Their specificity is often confirmed using knockout controls or peptide blocking assays. Researchers employ these tools to investigate CaMKIIα's role in neurodevelopmental disorders, neurodegenerative diseases (e.g., Alzheimer's), and psychiatric conditions linked to synaptic dysfunction. Notably, CaMKIIα antibodies also serve as markers for neuronal maturation, as its expression correlates with synaptic connectivity in developing brains. Commercial availability from multiple suppliers (e.g., MilliporeSigma, Abcam, Cell Signaling) and standardized validation protocols have made them indispensable for probing activity-dependent neural signaling pathways.