CathepsinB抗体;CathepsinB Antibody—艾普蒂

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     Western blot analysis of Cathepsin B expression in A375 cell lysate;.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

Cathepsin B is a lysosomal cysteine protease belonging to the papain family, primarily involved in intracellular protein degradation and processing. It plays critical roles in normal cellular processes such as apoptosis, antigen presentation, and tissue remodeling. However, dysregulated Cathepsin B expression or activity has been implicated in various pathological conditions, including cancer metastasis, neurodegenerative diseases (e.g., Alzheimer’s), inflammatory disorders, and infectious diseases. Its overexpression in tumors correlates with enhanced invasion, angiogenesis, and chemotherapy resistance, making it a potential therapeutic target and biomarker.

Antibodies targeting Cathepsin B are essential tools for studying its expression, localization, and function in both physiological and disease contexts. These antibodies enable detection through techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specificity validation is crucial, as Cathepsin B shares structural homology with other cathepsin family members. High-quality antibodies help distinguish between the inactive pro-enzyme (∼40 kDa) and active mature forms (∼25-30 kDa), providing insights into its activation status. Researchers also use these antibodies to explore therapeutic strategies, such as inhibiting Cathepsin B in cancer or modulating its activity in lysosomal storage disorders. Cross-reactivity with orthologs in common model organisms (e.g., mouse, rat) further supports translational studies. Overall, Cathepsin B antibodies are vital for advancing research into its dual roles in health and disease.