Rhodopsin抗体;Rhodopsin Antibody—艾普蒂 新品

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     Western blot analysis of Rhodopsin expression in rat eyeball lysate.    

  
  

Rhodopsin antibodies are essential tools in vision research, primarily targeting rhodopsin, a light-sensitive G protein-coupled receptor (GPCR) found in rod cells of the retina. Rhodopsin plays a critical role in phototransduction, converting light into electrical signals for dim-light vision. Structurally, it consists of an opsin protein bound to a retinal chromophore. Mutations in the RHO gene encoding rhodopsin are linked to inherited retinal diseases like retinitis pigmentosa (RP), making rhodopsin a key focus in studying degenerative eye disorders.

Rhodopsin antibodies are widely used to detect, localize, and quantify rhodopsin expression in retinal tissues, cell cultures, or experimental models. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence, aiding in the analysis of protein distribution, post-translational modifications, and disease-associated misfolding. These antibodies often target specific epitopes, such as the C-terminal region critical for intracellular signaling or extracellular loops involved in ligand binding.

Additionally, rhodopsin antibodies help investigate autoimmune retinopathies, where anti-rhodopsin autoantibodies may contribute to vision loss. Researchers also use them to validate gene-editing therapies or disease models (e.g., transgenic mice). Commercial antibodies are typically raised in rabbits or mice, with validation ensuring specificity for reduced cross-reactivity. Overall, rhodopsin antibodies bridge molecular insights into retinal physiology and pathology, supporting both basic research and therapeutic development for vision-related diseases.