TRF1抗体;TRF1 Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of TRF1 expression in HeLa cell lysate.    

  
  

TRF1 (Telomeric Repeat Binding Factor 1) is a key component of the shelterin complex, which safeguards telomeres—the protective caps at chromosome ends. TRF1 binds directly to double-stranded telomeric DNA repeats (TTAGGG)n, facilitating the formation of T-loop structures that prevent telomere degradation and end-to-end fusion. It plays a critical role in regulating telomere length by recruiting helicases and other enzymes to control replication stress and inhibit excessive elongation by telomerase. Dysregulation of TRF1 is linked to genomic instability, accelerated aging, and cancer progression.

TRF1 antibodies are essential tools for studying telomere biology. They enable the detection and localization of TRF1 in techniques like immunofluorescence, Western blotting, and chromatin immunoprecipitation (ChIP). These antibodies help investigate TRF1’s interactions with shelterin partners (e.g., TIN2. POT1) and its role in telomere maintenance, replication fork dynamics, and cellular senescence. Commercially available TRF1 antibodies are often validated for specificity across species (human, mouse, rat) and applications. Researchers use them to explore TRF1’s involvement in diseases, such as cancers with telomere dysfunction, or to assess telomere-related responses to therapies. Proper controls, like siRNA knockdown or TRF1-deficient cells, are recommended to confirm antibody specificity in experimental settings.