DUSP1抗体;DUSP1 Antibody—艾普蒂

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of DUSP1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate.    

  
  

DUSP1 (Dual Specificity Phosphatase 1), also known as MAPK phosphatase 1 (MKP-1), is a member of the dual-specificity phosphatase family that negatively regulates mitogen-activated protein kinase (MAPK) signaling pathways by dephosphorylating and inactivating MAPKs, including ERK, JNK, and p38. This enzyme plays a critical role in controlling cellular responses to stress, inflammation, and growth signals, making it a key modulator of immune function, apoptosis, and cell proliferation. Dysregulation of DUSP1 has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.

DUSP1 antibodies are essential tools for studying its expression, localization, and functional interactions in biological systems. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify protein levels in tissues or cultured cells under different experimental conditions. Researchers also employ these antibodies to explore DUSP1's role in feedback mechanisms that limit excessive MAPK activation, as well as its interplay with signaling pathways influenced by glucocorticoids or oxidative stress. Commercially available antibodies are typically validated for specificity using knockout controls or siRNA-mediated silencing. However, variations in isoform expression or post-translational modifications may affect detection accuracy, necessitating careful experimental optimization. Studies using DUSP1 antibodies have contributed to understanding its dual role as both a tumor suppressor and promoter, depending on cellular context and disease stage.