HistoneH3抗体;HistoneH3 Antibody—艾普蒂

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     Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) Rat brain lysate.    

  
  

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     Histone methyltransferase NSD2 regulates apoptosis and chemosensitivity in osteosarcoma. -Cell Death and Disease    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody at 1:200 dilution.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat pancreas, using the Antibody at 1:200 dilution.    

  
  

Histone H3 antibodies are essential tools in epigenetics and chromatin biology research, targeting histone H3—a core component of nucleosomes. Histones, including H3. play a critical role in DNA packaging and regulating gene expression through post-translational modifications (PTMs) such as acetylation, methylation, and phosphorylation. Histone H3 exists in multiple variants (e.g., H3.1. H3.3. CENP-A), each contributing to chromatin dynamics in distinct cellular processes. Antibodies against Histone H3 are widely used to investigate chromatin structure, epigenetic regulation, and cellular processes like DNA repair, replication, and mitosis.

These antibodies often target specific PTM sites or variants. For example, anti-H3K4me3 identifies trimethylation at lysine 4 (associated with active transcription), while anti-H3K27me3 detects repressive chromatin marks. Others recognize pan-H3 epitopes, serving as loading controls in Western blotting. Their applications span chromatin immunoprecipitation (ChIP), immunofluorescence, and flow cytometry.

Quality and specificity are critical, as cross-reactivity with other histones or non-histone proteins can lead to misinterpretation. Validated antibodies are vital for studying diseases linked to histone dysregulation, such as cancer, neurodegeneration, and developmental disorders. Recent advances in genome-wide profiling (e.g., ChIP-seq) further underscore their importance in mapping epigenetic landscapes and therapeutic target discovery.