Occludin抗体;Occludin Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

Occludin is a key transmembrane protein component of tight junctions, specialized cell-cell adhesion structures that regulate paracellular permeability and maintain polarity in epithelial and endothelial cells. Discovered in the early 1990s, occludin interacts with other junctional proteins like claudins and ZO-1 to form dynamic barriers controlling solute transport, cell signaling, and tissue integrity. Occludin antibodies are essential tools for studying tight junction organization and function in physiological and pathological contexts, including cancer, inflammation, and neurological disorders. These antibodies enable the detection of occludin expression, localization, and post-translational modifications (e.g., phosphorylation) via techniques like Western blotting, immunofluorescence, and immunohistochemistry.

Structurally, occludin contains four transmembrane domains, two extracellular loops, and cytoplasmic N- and C-termini. Its C-terminal region interacts with cytoskeletal adaptors, making epitope specificity critical for antibody performance. Researchers often validate occludin antibodies using knockout cell lines or tissues to confirm selectivity. Species cross-reactivity (human, mouse, rat) is a key consideration in experimental design. Both monoclonal and polyclonal antibodies are available, with monoclonal antibodies offering higher specificity and polyclonal antibodies capturing broader epitopes. Dysregulation of occludin expression correlates with barrier dysfunction in diseases like inflammatory bowel disease and Alzheimer's, underscoring the antibody's utility in mechanistic and therapeutic research.