Cdc27/APC3抗体;Cdc27/APC3 Antibody—艾普蒂

The Cdc27/APC3 antibody targets a key subunit of the anaphase-promoting complex/cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase essential for cell cycle progression. APC/C regulates the transition from metaphase to anaphase by tagging specific proteins (e.g., securin, cyclins) with ubiquitin, marking them for proteasomal degradation. Cdc27 (APC3) is a conserved TPR (tetratricopeptide repeat)-containing subunit critical for APC/C assembly and substrate recognition. Antibodies against Cdc27/APC3 are widely used to study cell cycle dynamics, particularly in mitosis and meiosis. They help detect APC/C composition, localization, and activity via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Research using these antibodies has clarified APC/C's role in checkpoint control, genomic stability, and cancer (where dysregulated APC/C activity is linked to tumorigenesis). Cdc27 itself was initially identified in yeast (Cdc27) and later in mammals, with antibodies aiding cross-species functional studies. These tools remain pivotal in exploring how APC/C dysfunction contributes to diseases, drug responses, or developmental defects.