MEKK3抗体;MEKK3 Antibody—艾普蒂 新品

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     Western blot analysis of MEKK3 expression in Hela cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

The MEKK3 (Mitogen-Activated Protein Kinase Kinase Kinase 3) antibody is a critical tool for studying the MEKK3 protein, a serine/threonine kinase within the MAP3K family. MEKK3 plays a pivotal role in regulating multiple signaling pathways, including the NF-κB, ERK, and p38 MAPK cascades, which are involved in cellular responses to stress, inflammation, proliferation, and apoptosis. It acts upstream of downstream kinases, mediating signals from cell surface receptors to intracellular targets. Dysregulation of MEKK3 has been implicated in various pathologies, such as cardiovascular diseases, cancer, and immune disorders, making it a focus of biomedical research.

MEKK3 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect MEKK3 expression, localization, and post-translational modifications. These antibodies help elucidate MEKK3’s interaction partners, activation mechanisms, and tissue-specific roles. For instance, studies have revealed MEKK3’s involvement in angiogenesis, embryonic development, and cytokine signaling. Researchers often validate antibody specificity using knockout cell lines or siRNA-mediated MEKK3 depletion to ensure accurate results.

Commercially available MEKK3 antibodies are typically raised against specific epitopes, such as the N-terminal or kinase domains, and vary in host species (e.g., rabbit, mouse). Selecting the right antibody depends on the experimental application and species reactivity. Proper controls and optimization are essential due to potential cross-reactivity with homologous kinases like MEKK2. Overall, MEKK3 antibodies are indispensable for advancing our understanding of cellular signaling networks and their implications in health and disease.