AMPKbeta1抗体;AMPKbeta1 Antibody—艾普蒂

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     Western blot analysis of AMPK beta 1 expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

AMPK beta1 antibody is a key tool used to study the beta1 regulatory subunit of AMP-activated protein kinase (AMPK), a crucial energy sensor in eukaryotic cells. AMPK is a heterotrimeric enzyme composed of α (catalytic), β, and γ subunits, with β subunits (beta1 and beta2) serving structural roles by anchoring the complex and regulating enzyme activity. The beta1 subunit (encoded by PRKAB1) is ubiquitously expressed and facilitates AMPK assembly, stability, and interaction with glycogen via its carbohydrate-binding module.

Antibodies targeting AMPK beta1 are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions in tissues or cells. They help investigate AMPK's role in metabolic regulation, including glucose uptake, lipid metabolism, and mitochondrial homeostasis. Researchers also employ these antibodies to study AMPK dysregulation in diseases like diabetes, obesity, cancer, and neurodegenerative disorders.

Specificity is critical, as beta1 shares homology with beta2; thus, high-quality antibodies are validated to minimize cross-reactivity. These reagents are essential for elucidating isoform-specific functions and signaling pathways, particularly in tissue-specific knockout models. Commercial AMPK beta1 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant proteins, with validation in knockdown/knockout controls. Their application supports translational research aiming to develop AMPK-targeted therapies for metabolic and age-related diseases.