14-3-3sigma抗体;14-3-3sigma Antibody—艾普蒂

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of 14-3-3 sigma expression in A431 cell lysate.    

  
  

The 14-3-3σ protein, also known as stratifin, is a member of the evolutionarily conserved 14-3-3 family of regulatory proteins, which comprises seven isoforms (β, γ, ε, η, σ, τ/θ, ζ) in humans. These proteins function as adaptors that modulate interactions between phosphorylated client proteins, influencing diverse cellular processes such as cell cycle regulation, apoptosis, signal transduction, and DNA damage response. The 14-3-3σ isoform is distinct for its epithelial-specific expression and role in maintaining cellular homeostasis. It is transcriptionally regulated by p53 and plays a key role in the G2/M checkpoint control by sequestering cyclin-dependent kinase complexes in response to DNA damage, thereby preventing mitotic progression in damaged cells.

14-3-3σ is implicated in tumor suppression, with reduced expression observed in multiple cancers (e.g., breast, lung, prostate) due to promoter hypermethylation. However, its role is context-dependent, as overexpression has also been linked to chemoresistance and poor prognosis in certain malignancies. Antibodies targeting 14-3-3σ are essential tools for studying its expression, localization, and interactions in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to explore its involvement in cancer biology, therapeutic resistance, and as a potential biomarker. Research on 14-3-3σ continues to uncover its dual roles in tumorigenesis and cellular stress responses.