E2F2抗体;E2F2 Antibody—艾普蒂

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     Western blot analysis of E2F2 expression in K562 cell lysate.    

  
  

The E2F2 antibody is a crucial tool in studying the E2F family of transcription factors, which play pivotal roles in cell cycle regulation, apoptosis, and differentiation. E2F2. a member of the E2F family, primarily functions as a transcriptional activator that drives the expression of genes essential for G1/S phase transition, including cyclin E and CDK2. It binds to target promoters through conserved DNA-binding domains and collaborates with DP proteins to regulate cell proliferation. E2F2 is particularly associated with the activation of quiescent cells into the cell cycle, and its dysregulation has been implicated in cancer development, where overexpression may lead to uncontrolled proliferation or genomic instability.

E2F2 antibodies are widely used in research to detect and quantify E2F2 protein levels in various experimental models, such as Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry. These antibodies help elucidate E2F2's spatial-temporal expression patterns, post-translational modifications, and interactions with regulatory proteins like retinoblastoma (Rb) family members. Studies using E2F2-deficient mice have highlighted its role in hematopoiesis and lymphocyte function, further underscoring its biological significance. Commercially available E2F2 antibodies are typically validated for specificity against conserved epitopes, though cross-reactivity with other E2F family members (e.g., E2F1 or E2F3) remains a consideration. Their applications extend to cancer research, where E2F2 expression profiles are analyzed in tumor tissues to assess prognostic or therapeutic relevance.