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网站主页 HistoneH3(dimethylK9)抗体 HistoneH3(dimethylK9)抗体
  • HistoneH3(dimethylK9)抗体;HistoneH3(dimethylK9) Antibody—艾普蒂
  • HistoneH3(dimethylK9)抗体;HistoneH3(dimethylK9) Antibody—艾普蒂
  • HistoneH3(dimethylK9)抗体;HistoneH3(dimethylK9) Antibody—艾普蒂

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HistoneH3(dimethylK9)抗体;HistoneH3(dimethylK9) Antibody—艾普蒂

Rabbit Monoclonal HistoneH3(dimethylK9) Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-29

上海切尔齐生物科技有限公司

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联系人：周经理
电话：027-19371278702拨打
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邮箱：nuodina88@163.com

产品详情:

中文名称：
HistoneH3(dimethylK9)抗体
英文名称：
Rabbit Monoclonal HistoneH3(dimethylK9) Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 7943 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Rabbit
偶联物：
无
靶点：
HistoneH3(dimethylK9)

验证与应用

应用及物种
WB1/1000-1/2000 Human,Mouse,Rat
IFChIP:1/20-1/50 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

Aliases

Histone H3.1; Histone H3; HIST1H3A; H3K9me2;

;DiMethyl-Histone H3 (K9)
WB Predicted band size15 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
Immunogen

A synthesized peptide derived from human Histone H3.1 around the methylation site of K80

FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       Western blot analysis of Histone H3 (di methyl K9) expression in HeLa cell lysate.    


           

参考文献

以下是三篇涉及Histone H3K9me2(dimethylK9)抗体的代表性文献，内容涵盖其应用方向及研究意义：

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1. **文献名称**：*Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain*

**作者**：Bannister AJ, et al.

**摘要**：该研究首次揭示HP1蛋白通过其chromo结构域特异性识别H3K9me2/me3修饰，并阐明了该修饰在异染色质形成及基因沉默中的关键作用。实验中采用H3K9me2抗体进行染色质免疫沉淀(ChIP)验证结合位点。

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2. **文献名称**：*Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer*

**作者**：Fraga MF, et al.

**摘要**：研究通过H3K9me2及H4K16ac等抗体进行免疫组化分析，发现多种癌症中H3K9me2的异常分布与表观遗传失调相关，提示其作为肿瘤生物标志物的潜力。

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3. **文献名称**：*Genome-wide maps of histone modifications in pluripotent and lineage-committed cells*

**作者**：Mikkelsen TS, et al.

**摘要**：通过ChIP-seq结合H3K9me2抗体，绘制多能干细胞与分化细胞的全基因组组蛋白修饰图谱，揭示H3K9me2在维持染色质动态平衡及细胞命运决定中的调控网络。

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**备注**：上述文献均发表于高影响力期刊(如Nature、Cell)，实验中使用H3K9me2抗体作为核心工具，建议通过PubMed或DOI进一步获取全文。如需更多方向(如神经疾病、衰老等)，可补充说明。

       

背景信息

Histone H3 (dimethylK9) antibodies are essential tools for studying epigenetic regulation and chromatin dynamics. Histone H3 lysine 9 dimethylation (H3K9me2) is a post-translational modification associated with transcriptional repression and heterochromatin formation. This mark is primarily deposited by histone methyltransferases such as G9a/GLP and SUV39H1. which play roles in gene silencing, X-chromosome inactivation, and maintaining genomic stability. Unlike H3K9me3. which is strongly linked to constitutive heterochromatin, H3K9me2 is enriched in facultative heterochromatin and participates in dynamic gene regulation during development and differentiation.

These antibodies are widely used in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to investigate epigenetic mechanisms in diseases such as cancer, neurological disorders, and aging. For example, aberrant H3K9me2 patterns have been observed in various cancers, correlating with oncogene activation or tumor suppressor silencing. In stem cell research, they help assess pluripotency and differentiation states by mapping repressive chromatin domains.

Most commercial H3K9me2 antibodies are generated using synthetic peptides corresponding to the dimethylated H3K9 region, followed by affinity purification. Specificity validation typically includes peptide competition assays and knockout cell line controls. Researchers must optimize experimental conditions due to potential cross-reactivity with similar histone modifications (e.g., H3K27me2). Recent advancements in single-cell epigenomic techniques have further expanded applications of these antibodies in mapping chromatin landscapes at high resolution.

       
HistoneH3(dimethylK9;HistoneH3(dimethylK9;HistoneH3(dimethylK9;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

HistoneH3(dimethylK9)抗体相关厂家报价

  • 抗CRF抗体
  • 抗CRF抗体
  • 维百奥（北京）生物科技有限公司 VIP
  • 2025-12-29
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