AKAP14抗体;AKAP14 Antibody—艾普蒂

The AKAP14 antibody is a tool used to study the A-kinase anchoring protein 14 (AKAP14), a member of the AKAP family that localizes protein kinase A (PKA) to specific cellular compartments. AKAP14. also known as AKAP28. is involved in anchoring PKA to regulatory sites, facilitating cAMP-dependent signaling pathways. It is predominantly expressed in tissues like testes, ciliated epithelia, and skeletal muscle, suggesting roles in sperm motility, ciliary function, and muscle contraction. The antibody detects AKAP14 in applications such as Western blotting, immunofluorescence, or immunoprecipitation, aiding in the investigation of its spatial-temporal regulation and interactions with PKA isoforms or other signaling components. Researchers utilize it to explore AKAP14's involvement in physiological processes (e.g., spermatogenesis, airway cilia activity) or pathological conditions (e.g., infertility, respiratory disorders). Validation often includes testing in AKAP14-knockout models or siRNA-treated cells to confirm specificity. Commercial antibodies may target specific epitopes, such as the N-terminal or central regions, and cross-reactivity with other AKAPs should be assessed. Its utility extends to both basic research and clinical studies, particularly in reproductive health and ciliopathy-related diseases.