PBP抗体;PBP Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Immunofluorescent analysis of Hela cells, using PEBP1 Antibody.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human liver, using PEBP1 Antibody.    

  
  

Penicillin-binding protein (PBP) antibodies are immunological tools used to study PBPs, a group of bacterial enzymes critical for peptidoglycan synthesis during cell wall formation. PBPs bind β-lactam antibiotics (e.g., penicillin) via their active sites, a mechanism exploited by these drugs to inhibit cell wall assembly and kill bacteria. However, antibiotic resistance often arises from PBP modifications, such as mutations or acquisition of alternative PBPs (e.g., PBP2a in methicillin-resistant *Staphylococcus aureus* [MRSA]), which reduce drug binding affinity.

PBP antibodies are developed to detect and characterize these proteins in bacterial pathogens. They aid in understanding resistance mechanisms, strain typing, and epidemiological surveillance. For example, anti-PBP2a antibodies are used in rapid diagnostic assays to confirm MRSA infections by identifying the presence of the *mecA*-encoded PBP2a. Similarly, antibodies against PBPs in *Streptococcus pneumoniae* help track vaccine escape strains with altered PBPs.

These antibodies are typically generated through immunization with recombinant PBP fragments or synthetic peptides. They enable techniques like Western blotting, ELISA, and immunofluorescence to study PBP expression, localization, and interaction with antibiotics. Recent research also explores PBP-targeting monoclonal antibodies as potential therapeutics to enhance β-lactam efficacy against resistant strains. Overall, PBP antibodies serve as vital reagents in both basic microbiology and clinical diagnostics, bridging antibiotic discovery and resistance management.