Tuberin抗体;Tuberin Antibody—艾普蒂

+ +

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

•  

   

     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

•  

   

     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Tuberin Antibody.    

  
  

Tuberin, encoded by the *TSC2* gene, is a critical tumor suppressor protein that forms a complex with Hamartin (TSC1) to regulate cell growth and metabolism. This TSC1-TSC2 complex inhibits the mechanistic target of rapamycin (mTOR) signaling pathway by acting as a GTPase-activating protein (GAP) for Rheb (Ras homolog enriched in brain), thereby suppressing mTORC1-mediated anabolic processes. Dysregulation of Tuberin due to pathogenic mutations in *TSC2* is linked to tuberous sclerosis complex (TSC), a genetic disorder characterized by benign tumor formation in multiple organs.

Antibodies targeting Tuberin are essential tools in both research and diagnostics. They enable the detection of Tuberin expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate the functional status of the TSC1-TSC2 complex, assess post-translational modifications (e.g., phosphorylation at Ser939 or Thr1462. which modulate its activity), and investigate mTOR pathway dysregulation in TSC-related pathologies. Additionally, they are used to study crosstalk between Tuberin and other signaling networks, such as PI3K/AKT, in cancer and metabolic diseases. Commercially available Tuberin antibodies vary in specificity, with validation often including knockout cell lines or tissue controls to ensure reliability. Their application extends to preclinical studies evaluating mTOR-targeted therapies, making them pivotal in advancing understanding of TSC and mTOR-driven disorders.