HistoneH3.3抗体;HistoneH3.3 Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Immunohistochemical analysis of paraffin-embedded mouse stomach, using Histone H3.3 Antibody .    

  
  

Histone H3.3 is a replication-independent histone variant encoded by two distinct genes, *H3F3A* and *H3F3B*, which replace canonical histones H3.1 and H3.2 in a DNA synthesis-independent manner. Unlike its canonical counterparts, H3.3 is incorporated into chromatin throughout the cell cycle, particularly at transcriptionally active regions, regulatory elements (e.g., promoters, enhancers), and heterochromatic sites like telomeres. Its dynamic deposition is mediated by chaperones such as HIRA and DAXX/ATRX, enabling roles in epigenetic regulation, transcriptional activation, and genome stability.

H3.3-specific antibodies are critical tools for studying its localization, function, and involvement in cellular processes such as differentiation, development, and aging. These antibodies typically target unique residues or post-translational modifications (PTMs) that distinguish H3.3 from H3.1/H3.2. For example, H3.3 contains amino acid substitutions at positions 87 (alanine vs. serine in H3.1) and 90 (glycine vs. methionine), enabling selective detection. Researchers use these antibodies in techniques like ChIP-seq, immunofluorescence, and Western blotting to explore H3.3's role in gene regulation, DNA repair, and disease contexts, including cancer and neurodevelopmental disorders.

However, antibody specificity must be rigorously validated due to high sequence similarity among H3 variants and PTM cross-reactivity. H3.3 dysregulation has been linked to gliomas, pediatric bone cancers, and other malignancies, underscoring the antibody's utility in both basic and translational research. Its application continues to advance our understanding of chromatin dynamics and epigenetic mechanisms in health and disease.