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网站主页 DGCR8 antibody DGCR8抗体
  • DGCR8抗体;DGCR8 Antibody—艾普蒂
  • DGCR8抗体;DGCR8 Antibody—艾普蒂
  • DGCR8抗体;DGCR8 Antibody—艾普蒂

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DGCR8抗体;DGCR8 Antibody—艾普蒂

Rabbit Monoclonal DGCR8 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-29

上海切尔齐生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：nuodina88@163.com

产品详情:

中文名称：
DGCR8抗体
英文名称：
Rabbit Monoclonal DGCR8 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 7890 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Rabbit
偶联物：
无
靶点：
DGCR8

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20-1/50 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesDGCR8; DGCRK6; Gy1 ; C22orf12; D16Wis2; pasha;;DGCR8
WB Predicted band sizeCalculated MW: 86 kDa ; Observed MW: 100 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human DGCR8
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    


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       Immunofluorescent analysis using the Antibody at 1:50 dilution.    


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       Immunofluorescent analysis using the Antibody at 1:50 dilution.    


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       Immunofluorescent analysis using the Antibody at 1:150 dilution.    


           

参考文献

以下是关于DGCR8抗体的3篇代表性文献的简要信息(注：文献信息为模拟示例，实际引用需以真实文献为准)：

1. **文献名称**: "DGCR8 is essential for microRNA biogenesis and silencing in embryonic stem cells"

**作者**: Wang Y, et al.

**摘要**: 本研究利用DGCR8特异性抗体验证了DGCR8蛋白在胚胎干细胞中的表达及定位，发现其缺失导致microRNA加工异常，并影响干细胞多能性维持。

2. **文献名称**: "A novel role for DGCR8 in psychiatric disorders linked to 22q11.2 deletion"

**作者**: Stark KL, et al.

**摘要**: 通过Western blot和免疫组化实验，作者使用DGCR8抗体发现22q11.2缺失综合征患者脑组织中DGCR8蛋白水平降低，提示其与神经发育异常相关。

3. **文献名称**: "Interaction between DGCR8 and Drosha revealed by co-immunoprecipitation assays"

**作者**: Lee Y, et al.

**摘要**: 该研究利用DGCR8抗体进行免疫共沉淀实验，证实DGCR8与Drosha形成复合体，并解析了其在microRNA初级转录本加工中的协同作用。

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如需真实文献，建议在PubMed或Google Scholar中搜索关键词 "DGCR8 antibody" + "microRNA" / "co-immunoprecipitation" / "knockout" 等，筛选近年高引论文。

       

背景信息

DGCR8 (DiGeorge Syndrome Critical Region Gene 8) is a key protein involved in the early stages of microRNA (miRNA) biogenesis. It forms a heterodimeric complex with the RNase III enzyme Drosha, known as the Microprocessor complex, which cleaves primary miRNA transcripts (pri-miRNAs) into precursor miRNAs (pre-miRNAs) in the nucleus. This step is critical for miRNA maturation and subsequent gene regulation. DGCR8 recognizes pri-miRNA structures through its RNA-binding domains and stabilizes Drosha for precise cleavage.

Antibodies targeting DGCR8 are essential tools for studying miRNA processing mechanisms, protein localization, and expression dynamics. They are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate DGCR8's interactions, cellular distribution, and regulatory roles. Researchers also employ these antibodies to explore DGCR8 dysfunction in diseases, such as DiGeorge syndrome (linked to 22q11.2 deletions), neurodevelopmental disorders, and cancers, where miRNA dysregulation is implicated.

Validated DGCR8 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and undergo rigorous testing for specificity (e.g., using knockout cell lines). Their applications span basic research on RNA biology and translational studies aiming to dissect miRNA-related pathologies or therapeutic targets.

       
DGCR8抗体;DGCR8;DGCR8 Antibody;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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