Phospho-HistoneH1.4(T17)抗体;Phospho-HistoneH1.4(T17) Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Phospho-Histone H1.4 (T17) expression in Jurkat cell lysate.    

  
  

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     Immunohistochemical analysis of paraffin-embedded mouse colon, using Phospho-Histone H1.4 (T17) Antibody.    

  
  

The Phospho-Histone H1.4 (T17) antibody is a specialized tool used to detect the phosphorylation of histone H1.4 at threonine 17. a post-translational modification linked to chromatin dynamics and cellular processes. Histone H1.4. a linker histone, stabilizes higher-order chromatin structure and regulates gene expression by binding to nucleosomes. Phosphorylation of H1.4 at T17 is associated with chromatin relaxation during the cell cycle, particularly in mitosis or DNA damage response, facilitating access to repair machinery or transcriptional activation. This modification may also play a role in apoptosis and cellular differentiation.

The antibody is commonly applied in techniques like Western blotting, immunofluorescence, or chromatin immunoprecipitation (ChIP) to study H1.4 phosphorylation dynamics in models of cancer, stem cell biology, or genotoxic stress. Researchers use it to explore how T17 phosphorylation correlates with cell cycle progression (e.g., G2/M transition), DNA damage pathways (e.g., ATM/ATR signaling), or epigenetic reprogramming. Specificity validation often includes knockout/knockdown controls or phosphatase treatment to confirm phospho-dependence.

Understanding H1.4(T17) phosphorylation provides insights into chromatin remodeling mechanisms and disease-related dysregulation, making this antibody valuable for basic and translational research in epigenetics and oncology.