ERK1/2抗体;ERK1/2 Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Polysaccharides from Nostoc commune Vaucher activate macrophages via NF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growth in vivo. -Food & Function    

  
  

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     Critical role for non‑GAP function of Gαs in RGS1‑mediated promotion of melanoma progression through AKT and ERK phosphorylation. -Oncology  Reports    

  
  

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     CircDLST promotes the tumorigenesis and metastasis of gastric cancer by sponging miR-502-5p and activating the NRAS/MEK1/ERK1/2 signaling. -Molecular Cancer    

  
  

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     Immunofluorescent analysis of Hela cells, using ERK1/2 Antibody.    

  
  

ERK1/2 (Extracellular Signal-Regulated Kinase 1/2), also known as MAPK3/MAPK1. are serine/threonine kinases central to the mitogen-activated protein kinase (MAPK) signaling pathway. They play critical roles in regulating cell proliferation, differentiation, survival, and apoptosis by transmitting signals from surface receptors to intracellular targets. ERK1 (44 kDa) and ERK2 (42 kDa) share ~85% sequence identity and are activated via dual phosphorylation at conserved threonine and tyrosine residues (Thr202/Tyr204 in ERK1; Thr185/Tyr187 in ERK2) by upstream MAPK/MEK kinases.

ERK1/2 antibodies are essential tools for studying this pathway’s activity and regulation. These antibodies are typically designed to detect either total ERK1/2 (regardless of activation status) or phosphorylated (activated) forms. Total ERK1/2 antibodies are used to assess protein expression levels, while phospho-specific antibodies help evaluate pathway activation in response to stimuli like growth factors, stress, or pharmacological inhibitors. Common applications include Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry.

Researchers frequently use ERK1/2 antibodies in cancer, neuroscience, and developmental biology studies, as dysregulated ERK signaling is implicated in malignancies, neurodegenerative diseases, and developmental disorders. Commercial ERK1/2 antibodies are often validated using knockout cell lines or siRNA-mediated knockdown to ensure specificity. Popular clones include those recognizing conserved epitopes, such as Clone 137F5 (Cell Signaling Technology) or Clone D13.14.4E (recognizing both ERK1 and ERK2). Proper controls, including stimulation with phorbol esters or EGF, are recommended to confirm antibody performance in detecting activation dynamics.