Apc6/CDC16抗体;Apc6/CDC16 Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Apc6 / CDC16 expression in HepG2 cell lysate.    

  
  

The Apc6/CDC16 antibody targets a conserved subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase critical for cell cycle regulation. APC/C orchestrates the transition from metaphase to anaphase by tagging specific proteins (e.g., securin, cyclins) with ubiquitin for proteasomal degradation, enabling sister chromatid separation and mitotic exit. The Apc6 subunit (also called CDC16 in yeast) contributes to APC/C assembly and substrate recognition, forming a tetratricopeptide repeat (TPR) domain-containing subcomplex with CDC23 and CDC27. which facilitates coactivator and substrate binding.

Apc6/CDC16 antibodies are widely used to study APC/C structure, dynamics, and function in mitosis, meiosis, and post-mitotic processes. They help detect APC/C localization, expression levels, or interactions via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Dysregulation of APC/C activity is linked to genomic instability, cancer, and developmental disorders, making these antibodies valuable tools for exploring cell cycle defects in disease models. Commercial Apc6/CDC16 antibodies are typically raised in rabbits or mice, validated for specificity across human, mouse, and other model organisms. Researchers often pair them with other APC/C subunit antibodies (e.g., CDC27. CDC23) to dissect complex regulation or screen for therapeutic targets in proliferative diseases.