IFIH1抗体;IFIH1 Antibody—艾普蒂

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     Cell lysate of THP-1 (untreated or treated with LPS) was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane.Result: P15671 can detect IFIH1 by Western blotting.    

  
  

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     Lane 1: LPS treated THP-1 lysate;  Lane 2: IFIH1 immunoprecipitated from LPS treated THP-1 lysate by P15671;  Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: P15671 can immunoprecipitate IFIH1;    

  
  

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     IHC-P analysis of thyroid gland tissue by IFIH1 antibody (P15671) IHC-P was performed using sections of the formalin-fixed paraffin-embedded thyroid gland tissue;Result: Follicular cells are positively stained at the cytoplasm.    

  
  

The IFIH1 gene encodes MDA5 (Melanoma Differentiation-Associated protein 5), a cytoplasmic RNA helicase and key component of the innate immune system. As a pattern recognition receptor, MDA5 detects viral double-stranded RNA, triggering interferon (IFN) production and antiviral responses. Dysregulation of IFIH1 is linked to autoimmune and inflammatory diseases. Gain-of-function mutations in IFIH1 cause Aicardi-Goutières syndrome (AGS), a severe neuroinflammatory disorder, while certain polymorphisms are associated with type 1 diabetes and systemic lupus erythematosus.

Autoantibodies against MDA5 (anti-MDA5) are clinically significant biomarkers, particularly in dermatomyositis (DM). These antibodies are strongly associated with clinically amyopathic DM and a high risk of rapidly progressive interstitial lung disease (RP-ILD), which carries poor prognosis. Detection of anti-MDA5 antibodies via ELISA or immunoprecipitation aids in diagnosis, risk stratification, and monitoring disease progression.

Research suggests MDA5 activation drives pathologic IFN signaling, making it a potential therapeutic target. JAK inhibitors and other IFN pathway modulators are being explored. Conversely, loss of MDA5 function increases susceptibility to viral infections. IFIH1 antibodies thus serve dual roles: as diagnostic tools in autoimmunity and as functional probes to study antiviral immunity. Their detection underscores the interplay between innate immunity and autoinflammation in human disease.