ARFGAP3抗体;ARFGAP3 Antibody—艾普蒂

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     Lane 1: Ramos lysate;  Lane 2: ARFGAP3 immunoprecipitated from Ramos by P15625;  Lane 3: The same  Lane 2 but use  IgG isotype control antibody ;Result: P15625 can immunoprecipitate ARFGAP3;    

  
  

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     IHC-P analysis of salivary gland tissue by ARFGAP3 antibody (P15625) IHC-P was performed using sections of the formalin-fixed paraffin-embedded salivary gland tissue;Result: Glandular cells are positively stained at the cytoplasm.    

  
  

**Background of ARFGAP3 Antibody**

ARFGAP3 (ADP-ribosylation factor GTPase-activating protein 3) is a member of the ARFGAP family, which regulates vesicular trafficking and membrane remodeling by accelerating GTP hydrolysis in ARF (ADP-ribosylation factor) proteins. ARFGAP3 primarily localizes to the Golgi apparatus and plays a role in COPI (coat protein complex I)-mediated retrograde transport, Golgi homeostasis, and lipid droplet formation. Its structure includes a conserved ARFGAP domain critical for GTPase activity and a GATA-like zinc finger domain involved in lipid binding.

Antibodies targeting ARFGAP3 are essential tools for studying its expression, intracellular localization, and interactions. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate ARFGAP3's regulatory mechanisms in cellular processes. Dysregulation of ARFGAP3 has been linked to cancers, neurodegenerative disorders, and metabolic diseases, making these antibodies valuable for exploring disease mechanisms.

Commercial ARFGAP3 antibodies are typically raised against specific epitopes, such as recombinant protein fragments or synthetic peptides. Researchers must validate antibody specificity using knockout controls or siRNA-mediated silencing. Applications often focus on ARFGAP3's role in secretory pathways, lipid metabolism, or its interplay with ARF isoforms (e.g., ARF1). Recent studies also highlight its potential as a therapeutic target, emphasizing the importance of reliable antibody reagents in both basic and translational research.