NAGK抗体;NAGK Antibody—艾普蒂

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     Lane 1: 15 µg of HeLa lysate;  Lane 2: 15 µg of Jurkat lysate;  Lane 3: 15 µg of HEK-293 lysate;Lane 4: 15 µg of A-431 lysate;  Result: P15566 can detect NAGK by Western blotting.    

  
  

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     Lane 1: HeLa lysate;  Lane 2: NAGK immunoprecipitated from HeLa lysate by P15566;  Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: P15566 can immunoprecipitate NAGK;    

  
  

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     IHC-P analysis of tonsil tissue by NAGK antibody (P15566) IHC-P was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue;Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm.    

  
  

NAGK (N-acetylglutamate kinase) is a mitochondrial enzyme encoded by the *NAGK* gene, primarily involved in the urea cycle by catalyzing the phosphorylation of N-acetylglutamate (NAG). NAGK-generated N-acetylglutamate phosphate serves as an essential allosteric activator of carbamoyl phosphate synthetase 1 (CPS1), a rate-limiting enzyme in urea synthesis. Dysregulation of NAGK has been linked to urea cycle disorders (UCDs), metabolic syndromes, and certain cancers, highlighting its physiological significance.

NAGK antibodies are immunological tools designed to detect and quantify NAGK protein expression in research and diagnostic contexts. These antibodies are typically produced using immunogenic peptides or recombinant NAGK proteins, yielding polyclonal or monoclonal variants with specificity validated via Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF). Applications include studying NAGK’s role in nitrogen metabolism, investigating urea cycle defects in liver diseases, and exploring its potential oncogenic functions in tumor models. Recent studies also implicate NAGK in autophagy and arginine metabolism, broadening its relevance in cellular homeostasis. Commercially available NAGK antibodies facilitate biomarker discovery and mechanistic research, though cross-reactivity and tissue-specific expression patterns require careful validation. Overall, NAGK antibodies are critical for advancing understanding of metabolic regulation and disease pathogenesis.