Mitf (phospho S157)抗体;Mitf (phospho S157) Antibody—艾普蒂 新品

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     IHC-P analysis of cutaneous melanoma tissue by Mitf antibody (P15373) IHC-P was performed using sections of the formalin-fixed paraffin-embedded cutaneous melanoma tissue;Result: Tumor cells are positively stained at the nuclei.    

  
  

The Mitf (phospho S157) antibody is a specialized tool used to detect the phosphorylation status of Microphthalmia-associated transcription factor (Mitf) at serine residue 157. Mitf is a critical transcription factor regulating melanocyte development, pigment production, and survival. Its activity is tightly controlled by post-translational modifications, including phosphorylation, which modulates its stability, subcellular localization, and transcriptional activity. Phosphorylation at Ser157 has been implicated in Mitf’s role in cell cycle regulation and differentiation. This site-specific phosphorylation may influence interactions with co-regulators or downstream target gene expression.

The Mitf (phospho S157) antibody enables researchers to study Mitf activation dynamics in response to signaling pathways, such as the MAPK/ERK cascade, which is often dysregulated in cancers like melanoma. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess Mitf activation in cell lines, tissue samples, or experimental models. Its specificity for the phosphorylated form allows differentiation between active and inactive Mitf states, providing insights into cellular responses to stimuli like UV exposure, growth factors, or stress signals.

This antibody is particularly valuable in melanoma research, as Mitf acts as both an oncogene and tumor suppressor depending on context. Dysregulated Mitf phosphorylation has been linked to tumor progression, drug resistance, and metastasis. By detecting p-Ser157 Mitf, researchers can explore its role in melanomagenesis, therapeutic response, and potential as a biomarker. Validation via knockout/knockdown controls or peptide competition is recommended to ensure specificity.