HEXIM1抗体;HEXIM1 Antibody—艾普蒂

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     IHC-P analysis of placenta tissue by HEXIM1 antibody (P15019) IHC-P was performed using sections of the formalin-fixed paraffin-embedded placenta tissue;Result: Decidual cells and trophoblastic cells are positively stained at the nuclei and cytoplasm.    

  
  

The HEXIM1 (hexamethylene bis-acetamide inducible protein 1) antibody is a crucial tool for studying the functional role of HEXIM1. a nuclear protein involved in transcriptional regulation. HEXIM1 is best known for its interaction with the 7SK small nuclear RNA (7SK snRNA) to form a ribonucleoprotein complex that sequesters and inactivates positive transcription elongation factor b (P-TEFb), a key regulator of RNA polymerase II-dependent transcription elongation. This interaction modulates gene expression by controlling the release of P-TEFb, which phosphorylates RNA polymerase II to promote transcriptional elongation. Dysregulation of HEXIM1 has been linked to diseases such as cancer, cardiac hypertrophy, and HIV latency, making it a target for therapeutic research.

HEXIM1 antibodies are widely used in molecular biology to detect HEXIM1 protein expression, localization, and interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. These antibodies help elucidate HEXIM1's dual roles as both a transcriptional suppressor (via P-TEFb inhibition) and an activator (in certain stress-responsive pathways). Researchers also employ HEXIM1 antibodies to study its post-translational modifications, such as phosphorylation, which influence its stability and function. Commercial HEXIM1 antibodies are typically raised against specific epitopes, often within its N-terminal or central regions, and validated for species cross-reactivity (e.g., human, mouse, rat). Proper validation using knockout controls is essential due to potential cross-reactivity with homologous proteins like HEXIM2.