TJP1抗体;TJP1 Antibody—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using ACP5 mouse mAb against JURKAT (1) and OCM-1 (2) cell lysate.    

  
  

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     Flow cytometric analysis of JURKAT cells using ACP5 mouse mAb (green) and negative control (red).    

  
  

TJP1 (Tight Junction Protein 1), also known as Zonula Occludens-1 (ZO-1), is a critical scaffolding protein localized at cellular tight junctions, specialized structures that regulate paracellular permeability and maintain cell polarity in epithelial and endothelial tissues. It belongs to the membrane-associated guanylate kinase (MAGUK) family, characterized by PDZ, SH3. and GuK domains that mediate interactions with transmembrane proteins (e.g., claudins, occludin) and cytoskeletal components. TJP1 plays a vital role in organizing tight junction assembly, signal transduction, and barrier integrity.

TJP1 antibodies are essential tools for studying tight junction dynamics in physiological and pathological contexts. They are widely used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to visualize TJP1 distribution, quantify expression levels, or assess junctional integrity in diseases such as cancer, inflammatory bowel disease, and skin disorders. Altered TJP1 expression or localization is associated with epithelial-mesenchymal transition (EMT), metastasis, and barrier dysfunction. Researchers also utilize these antibodies to explore therapeutic interventions targeting junctional stability or to validate in vitro models of epithelial barriers. Commercially available TJP1 antibodies are typically raised against specific epitopes (e.g., human C-terminal regions) and validated for cross-reactivity in common model organisms. Proper controls are crucial due to potential isoform variability and post-translational modifications affecting antibody specificity.