GTF3C4抗体;GTF3C4 Antibody—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-4: A172, Hela, Jurkat and Raji cell lysates, Primary antibody: P13544(GTF3C4 Antibody) at dilution 1/1300, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P13544(GTF3C4 Antibody) at dilution 1/160. (Original magnification: ×200)    

  
  

The GTF3C4 antibody targets the General Transcription Factor 3C Subunit 4 (GTF3C4), a protein component of the TFIIIC complex, which is essential for RNA polymerase III (Pol III)-mediated transcription. The TFIIIC complex binds to promoter regions of small non-coding RNAs, including tRNAs, 5S rRNA, and other Pol III-dependent transcripts, facilitating their transcription by recruiting the TFIIIB complex and positioning Pol III. GTF3C4. along with other subunits (GTF3C1-6), contributes to the structural and functional integrity of TFIIIC, with roles in DNA binding and promoter recognition.

Antibodies against GTF3C4 are primarily used in research to study Pol III transcription regulation, protein-protein interactions, and subcellular localization. They are applied in techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and co-immunoprecipitation (Co-IP) to investigate GTF3C4 expression, dynamics in cellular processes (e.g., cell division, stress responses), and potential dysregulation in diseases. Aberrant Pol III activity has been linked to cancers, viral infections, and genetic disorders, making GTF3C4 a subject of interest in understanding transcriptional malfunctions. Commercial GTF3C4 antibodies are validated for specificity and reproducibility, aiding in mechanistic studies of Pol III-driven transcription and its implications in pathology.