GTF3A抗体;GTF3A Antibody—艾普蒂

The GTF3A antibody targets the transcription factor III A (TFIIIA), a key protein involved in RNA polymerase III (Pol III)-mediated transcription. GTF3A, encoded by the GTF3A gene, is essential for the initiation of 5S ribosomal RNA (5S rRNA) synthesis, a critical component of ribosome assembly. TFIIIA binds directly to the internal promoter of 5S rRNA genes through its zinc finger domains, facilitating the recruitment of other Pol III-associated factors, such as TFIIIC and TFIIIB, to form a transcriptionally active complex. This process is tightly regulated and crucial for cellular protein synthesis, growth, and proliferation. Antibodies against GTF3A are widely used in molecular biology research to study Pol III-dependent transcription mechanisms, ribosome biogenesis, and their dysregulation in diseases like cancer or viral infections. They enable techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to analyze TFIIIA expression, localization, and DNA-binding activity. Commercial GTF3A antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers also utilize these antibodies to explore links between Pol III activity, cellular stress responses, and oncogenic pathways, offering insights into potential therapeutic targets for conditions marked by aberrant ribosome production or transcriptional dysfunction.