FOXH1抗体;FOXH1 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P13424(FOXH1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The FOXH1 antibody is a key tool for studying the Forkhead box H1 (FOXH1) protein, a member of the Forkhead box (FOX) family of transcription factors characterized by a conserved winged-helix DNA-binding domain. FOXH1 plays a critical role in early embryonic development, particularly in mediating signaling pathways such as TGF-β and Nodal. These pathways are essential for establishing the body plan, left-right asymmetry, and germ layer specification (e.g., mesoderm formation). FOXH1 acts as a transcriptional coactivator, binding to SMAD2/3-SMAD4 complexes to regulate target genes like *MIXL1* and *LEFTY2*.

FOXH1 antibodies are widely used in research to detect and quantify FOXH1 expression in models ranging from zebrafish and Xenopus to mammalian cells. They enable techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to explore FOXH1's localization, interactions, and regulatory mechanisms. Commercial FOXH1 antibodies are typically raised in rabbits or mice, with specificity validated via knockout controls or siRNA knockdown.

Dysregulation of FOXH1 has been linked to developmental disorders and cancers, including gastric and colorectal tumors, where it may promote metastasis. Researchers also utilize these antibodies in stem cell studies to assess pluripotency and differentiation. However, variability in antibody performance (e.g., cross-reactivity, batch differences) necessitates careful optimization of experimental conditions. Major suppliers include Abcam, Cell Signaling Technology, and Santa Cruz Biotechnology, each offering antibodies tailored for specific applications.