EPS15抗体;EPS15 Antibody—艾普蒂

The EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15) antibody is a tool used to study the EPS15 protein, a conserved adaptor protein involved in clathrin-mediated endocytosis and intracellular trafficking. First identified as a substrate of the epidermal growth factor receptor (EGFR), EPS15 plays a critical role in receptor internalization, signal transduction, and membrane dynamics. It contains three functional domains: an N-terminal EH domain for protein-protein interactions, a central coiled-coil region, and a C-terminal ubiquitin-interacting motif. Dysregulation of EPS15 has been linked to cancer progression, particularly in tumors with amplified EGFR or other receptor tyrosine kinases. The EPS15 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions in cellular pathways. Researchers employ this antibody to explore EPS15's role in endocytic sorting, cancer biology, and neurological disorders, as aberrant vesicular trafficking is implicated in diseases like Alzheimer's. Commercial EPS15 antibodies are typically raised against specific epitopes, such as the C-terminal region, and validated for cross-reactivity in human, mouse, and rat models.