EEA1抗体;EEA1 Antibody—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-4: NIH/3T3, RAW264.7 and 293T cell, Human metastatic malignant melanoma tissue lysates, Primary antibody: P13294(EEA1 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

The Early Endosome Antigen 1 (EEA1) antibody is a widely used tool in cell biology to study endosomal trafficking and membrane dynamics. EEA1 is a peripheral membrane protein localized to early endosomes, where it plays a critical role in endosome maturation, fusion, and cargo sorting. The protein contains an N-terminal Rab5-binding domain, a central coiled-coil region, and a C-terminal FYVE domain that binds phosphatidylinositol-3-phosphate (PI3P), anchoring it to early endosomal membranes.

EEA1 antibodies are primarily employed as markers for early endosomes in immunofluorescence, immunoelectron microscopy, and Western blotting. They help visualize the spatial organization of endosomes and track vesicular transport pathways in cellular processes like receptor internalization, autophagy, and pathogen entry. Dysregulation of EEA1-associated pathways has been linked to diseases, including cancer and neurodegenerative disorders, making these antibodies valuable in both basic research and translational studies.

Commercially available EEA1 antibodies are typically raised in rabbits or mice against specific epitopes, such as the C-terminal region. Validation includes testing for colocalization with Rab5 or other early endosome markers. Researchers must optimize protocols to avoid cross-reactivity with structurally similar proteins (e.g., Hrs or SARA) and ensure species compatibility. Overall, EEA1 antibodies remain indispensable for investigating intracellular trafficking mechanisms and their physiological or pathological implications.