CPSF2抗体;CPSF2 Antibody—艾普蒂

+ +

•  

   

     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P13215(CPSF2 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The Cleavage and Polyadenylation Specificity Factor subunit 2 (CPSF2), also known as CPSF100. is a critical component of the CPSF complex, which plays a central role in mRNA 3′-end processing. This protein is involved in the cleavage and polyadenylation of pre-mRNAs, essential steps for transcript maturation, stability, and nuclear export. CPSF2 specifically recognizes the polyadenylation signal (AAUAAA) in nascent RNA and recruits other processing factors to ensure accurate termination of RNA polymerase II transcription. Its function is conserved across eukaryotes, underscoring its importance in gene expression regulation.

CPSF2 antibodies are tools used to study the protein's expression, localization, and interactions in various biological contexts. Researchers employ these antibodies in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to investigate CPSF2's role in mRNA processing, cell cycle regulation, and development. Dysregulation of CPSF2 has been implicated in diseases, including cancers and viral infections (e.g., HIV), where altered mRNA processing contributes to pathogenesis. Antibodies against CPSF2 also aid in exploring its potential as a therapeutic target or biomarker. Commercial CPSF2 antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated depletion. However, users must optimize experimental conditions due to potential cross-reactivity with related proteins or isoforms. These reagents remain vital for dissecting the molecular mechanisms of mRNA maturation and its links to human diseases.