HIPK3抗体;HIPK3 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P13134(HIPK3 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The Homeodomain-Interacting Protein Kinase 3 (HIPK3) is a serine/threonine kinase belonging to the HIPK family, which includes four members (HIPK1-4). HIPK3 regulates diverse cellular processes, such as transcriptional regulation, stress signaling, cell proliferation, apoptosis, and DNA damage response. It interacts with transcription factors (e.g., p53. NF-κB) and modulates their activity through phosphorylation. HIPK3 is implicated in developmental pathways, tumorigenesis, and diseases like diabetes complications and neurodegenerative disorders. Its expression and activity are tightly regulated by post-translational modifications (PTMs) and cellular context.

HIPK3-specific antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are commonly developed against unique epitopes in its N-terminal or C-terminal regions, or specific phosphorylated residues. They enable applications like Western blotting, immunofluorescence, immunohistochemistry, and co-immunoprecipitation. Due to sequence homology among HIPK family members, antibody specificity must be rigorously validated using knockout cells or siRNA-mediated knockdown. Some commercial antibodies may cross-react with HIPK1 or HIPK2. necessitating careful experimental controls. HIPK3 antibodies have advanced research into its dual roles in promoting or suppressing tumors, depending on the cancer type, and its involvement in glucose metabolism and oxidative stress responses. Their utility extends to both basic research and potential clinical diagnostics, particularly in cancers and metabolic diseases.