ARFGEF1抗体;ARFGEF1 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P12949(ARFGEF1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P12949(ARFGEF1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

The ARFGEF1 (ADP-ribosylation factor guanine nucleotide-exchange factor 1) antibody is a tool used to detect and study the ARFGEF1 protein, also known as BIG1. This protein functions as a guanine nucleotide exchange factor (GEF) that activates ADP-ribosylation factors (ARFs), small GTPases critical for intracellular vesicular trafficking, Golgi maintenance, and cytoskeletal reorganization. ARFGEF1 facilitates the exchange of GDP for GTP on ARFs, enabling their participation in membrane recruitment of coat proteins and cargo sorting.

Research on ARFGEF1 has linked it to diverse cellular processes, including neurotransmitter release, hormone secretion, and cell signaling. It is implicated in diseases such as cancer, where altered expression may influence metastasis, and neurodevelopmental disorders, potentially through dysregulated synaptic vesicle transport. The ARFGEF1 antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression, localization, and interaction partners.

Developed in various host species (e.g., rabbit, mouse), these antibodies often target specific domains, such as the Sec7 catalytic domain. Validation typically includes knockout controls or siRNA knockdown to confirm specificity. Studying ARFGEF1 with such antibodies enhances understanding of its role in cellular homeostasis and pathology, offering insights for therapeutic targeting in trafficking-related diseases.