B4GAT1抗体;B4GAT1 Antibody—艾普蒂 新品

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue lysate, Primary antibody: P12935(B4GAT1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P12935(B4GAT1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

B4GAT1 (Beta-1.4-galactosyltransferase 1) is an enzyme encoded by the B4GAT1 gene, which plays a critical role in glycosylation processes. It catalyzes the transfer of galactose to terminal N-acetylglucosamine residues in glycoproteins and glycolipids, contributing to the synthesis of poly-N-acetyllactosamine chains—a key component of extracellular matrix structures. B4GAT1 is part of the glycosyltransferase 7 family and is notably involved in the post-translational modification of alpha-dystroglycan (α-DG), a protein essential for maintaining muscle membrane integrity and neuronal development. Dysregulation of B4GAT1-mediated glycosylation is linked to congenital disorders of glycosylation (CDGs), particularly Walker-Warburg syndrome and muscle-eye-brain disease, characterized by severe muscular dystrophy, brain malformations, and ocular defects.

Antibodies targeting B4GAT1 are vital tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in tissues or cell lines. Research utilizing B4GAT1 antibodies has advanced understanding of glycosylation defects in neuromuscular diseases and helped identify biomarkers for diagnostics. Additionally, these antibodies aid in exploring therapeutic strategies aimed at restoring glycosylation pathways. Commercial B4GAT1 antibodies are typically raised in rabbits or mice, with validation steps confirming specificity for human, mouse, or rat isoforms. Ongoing studies continue to refine their applications in mechanistic and translational research.