ZIC1抗体;ZIC1 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P12839(ZIC1 Antibody) at dilution 1/65. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P12839(ZIC1 Antibody) at dilution 1/65. (Original magnification: ×200)    

  
  

ZIC1 (Zinc Finger Protein of the Cerebellum 1) is a transcription factor belonging to the ZIC family, characterized by C2H2-type zinc finger domains. It plays critical roles in embryonic development, particularly in neural tube formation, cerebellar development, and neurogenesis. Dysregulation of ZIC1 has been linked to neurological disorders and cancers, including medulloblastoma and endometrial cancer.

ZIC1 antibodies are immunological tools designed to detect and quantify ZIC1 protein expression in research and diagnostic settings. These antibodies are typically generated using immunogenic peptides derived from conserved regions of the ZIC1 protein, such as the N-terminal or zinc finger domains. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to study ZIC1's expression patterns, subcellular localization, and DNA-binding activity.

In research, ZIC1 antibodies help elucidate its role in developmental biology and tumorigenesis. For example, they have been employed to identify ZIC1 overexpression in certain cancers, suggesting its potential as a biomarker or therapeutic target. Validation of ZIC1 antibodies includes testing specificity via knockout cell lines or siRNA silencing. Commercial ZIC1 antibodies vary in host species (rabbit, mouse) and clonality (monoclonal/polyclonal), with applications tailored to experimental needs. Proper controls are essential to ensure accurate interpretation, given potential cross-reactivity with other ZIC family members.