ULK1抗体;ULK1 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P12818(ULK1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The ULK1 (Unc-51-like kinase 1) antibody is a key tool for studying the molecular mechanisms of autophagy, a critical cellular process for maintaining homeostasis. ULK1. a serine/threonine kinase homologous to yeast Atg1. acts as a master initiator of autophagy under stress conditions. It forms a core complex with ATG13. FIP200. and ATG101. which integrates upstream signals from mTORC1 (inhibitory) and AMPK (activating) to regulate autophagosome formation. ULK1 phosphorylates downstream effectors like Beclin-1 and PI3KC3 to promote membrane nucleation.

Researchers use ULK1 antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect ULK1 expression, localization, and post-translational modifications. These antibodies help elucidate ULK1’s regulatory phosphorylation sites (e.g., Ser317/Ser777 by AMPK; Ser757 by mTOR) and its dysregulation in diseases. ULK1 is implicated in cancer (dual roles in tumor suppression/progression), neurodegenerative disorders (clearance of protein aggregates), and metabolic diseases. Commercial ULK1 antibodies are often validated for specificity against human/mouse isoforms, with some targeting phosphorylated forms to study activation states. Challenges include distinguishing ULK1 from its homolog ULK2 and detecting low-abundance endogenous proteins. Recent studies also explore ULK1’s non-autophagic roles in apoptosis, immune response, and vesicle trafficking, expanding its therapeutic relevance.