HAND1抗体;HAND1 Antibody—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue lysate, Primary antibody: P12816(HAND1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P12816(HAND1 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P12816(HAND1 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The HAND1 antibody is a crucial tool in developmental biology and cancer research, targeting the Heart and Neural Crest Derivatives-expressed protein 1 (HAND1), a basic helix-loop-helix (bHLH) transcription factor. HAND1 plays a pivotal role in embryonic development, particularly in cardiac morphogenesis, placental development, and differentiation of trophoblast cells. During embryogenesis, it regulates cell fate decisions, tissue patterning, and cell cycle exit in cardiac progenitor cells. Aberrant HAND1 expression is linked to congenital heart defects and placental insufficiency.

In research, the HAND1 antibody is widely used to detect HAND1 protein expression and localization via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It helps elucidate HAND1's role in transcriptional networks governing organogenesis. Notably, HAND1 is also implicated in cancer, with studies highlighting its overexpression in neuroblastoma and specific sarcomas, where it may serve as a diagnostic or prognostic marker.

Commercially available HAND1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein's N-terminal or bHLH domains. Validation methods, such as knockout controls, ensure specificity. Researchers must consider cross-reactivity risks with related proteins (e.g., HAND2) and tissue-specific expression patterns. Its utility extends to stem cell research, particularly in cardiac differentiation models, aiding regenerative medicine advancements. Overall, the HAND1 antibody remains indispensable for dissecting developmental pathways and disease mechanisms.